3NSF
Apo form of the multicopper oxidase CueO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.200, 90.810, 53.774 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 26.300 - 2.000 |
| R-factor | 0.19599 |
| Rwork | 0.193 |
| R-free | 0.25051 |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.377 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.300 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.350 |
| Number of reflections | 30343 | |
| <I/σ(I)> | 12.6 | 2.9 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6; , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
