3NSC
C500S MUTANT OF CueO BOUND TO Cu(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .9002 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.329, 91.574, 54.248 |
| Unit cell angles | 90.00, 102.48, 90.00 |
Refinement procedure
| Resolution | 20.300 - 1.500 |
| R-factor | 0.18223 |
| Rwork | 0.181 |
| R-free | 0.21253 |
| Structure solution method | UNnecessary |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.697 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.300 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.244 |
| Number of reflections | 72799 | |
| <I/σ(I)> | 10.1 | 1.9 |
| Completeness [%] | 95.0 | 99 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6; , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
