3NSB
Structure of bacteriorhodopsin ground state before and after X-ray modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.995, 60.995, 109.991 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.100 - 1.780 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.060 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.100 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 22171 | |
| <I/σ(I)> | 20.3 | 3.3 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | monoolein meso phase, K/Na-Pi as a precipitant, pH 5.6, temperature 293K |
