3NRY

Insights into anti-parallel microtubule crosslinking by PRC1, a conserved microtubule binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	173
Detector technology	CCD
Collection date	2009-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	.97918
Spacegroup name	P 62
Unit cell lengths	51.043, 51.043, 90.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.000
R-factor	0.23953
Rwork	0.238
R-free	0.27378
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.047
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.082	0.469
Number of reflections	17342
<I/σ(I)>	20.1	2
Completeness [%]	98.3	97.3
Redundancy	3.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	277	1:1 v/v protein (50 mg/ml in 80 mM PIPES, pH 6.8, 1 mM MgCl2, 1 mM EDTA, 150 mM KCl) and reservoir buffer (100 mM CHES pH 9.5, 30 % PEG 3000), VAPOR DIFFUSION, HANGING DROP, temperature 277K