3NRW

Crystal Structure of the N-terminal domain of Phage integrase/site-specific recombinase (tnp) from Haloarcula marismortui, Northeast Structural Genomics Consortium Target HmR208A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-30
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97901
Spacegroup name	P 61
Unit cell lengths	78.531, 78.531, 36.654
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.187
Rwork	0.186
R-free	0.20300
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX (followed by model building by Arp)
Refinement software	CNS (1.2 & XtalView)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.042	0.139
Number of reflections	23807
<I/σ(I)>	43.2	6.3
Completeness [%]	85.6	57.9
Redundancy	6.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5). Reservoir solution: 0.1M Sodium Acetate (pH 5), 2.99M NaCl, and 20% glycerol as cryo-protectant, Microbatch, under oil, temperature 277K