3NRT
The crystal structure of putative ryanodine receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.642, 89.272, 74.697 |
| Unit cell angles | 90.00, 110.99, 90.00 |
Refinement procedure
| Resolution | 32.480 - 2.540 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.24760 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.101 | 0.643 |
| <I/σ(I)> | 14 | 2.17 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 25% PEG 400, 0.2M MgCl, , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
