3NRO

Crystal Structure of putative transcriptional factor Lmo1026 from Listeria monocytogenes (FRAGMENT 52-321), Northeast Structural Genomics Consortium Target LmR194

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-03
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	44.236, 90.301, 118.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.660 - 2.900
R-factor	0.235
Rwork	0.234
R-free	0.26400
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.295
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Number of reflections	19967
<I/σ(I)>	19
Completeness [%]	98.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	293	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: LI2SO4 0.2M, Peg-4K 30%, 0.1M Tris, pH 8.5, macrobatch under oil, temperature 293K