3NRK
The crystal structure of the leptospiral hypothetical protein LIC12922
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2009-07-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 137.110, 137.110, 137.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.250 - 3.100 |
| R-factor | 0.2001 |
| Rwork | 0.199 |
| R-free | 0.21750 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.071 | 0.830 |
| Number of reflections | 15880 | |
| <I/σ(I)> | 21.4 | 2.61 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.3 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M sodium citrate pH 5.5, 1 M ammonium phosphate, 0.2 M sodium chloride, 0.2 % low melting point agarose, Microseeding, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
