3NR3

Crystal Structure of Human Peripheral Myelin Protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	67.190, 67.190, 100.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.920 - 1.950
R-factor	0.20793
Rwork	0.206
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pmp
RMSD bond length	0.011
RMSD bond angle	1.459
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.920	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.095	0.938
Number of reflections	17365
<I/σ(I)>	10.7	2
Completeness [%]	99.9	100
Redundancy	6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	2.5M ammonium_sulfate, Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 277K