3NQY
Crystal structure of the autoprocessed complex of Vibriolysin MCP-02 with a single point mutation E346A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-06-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.48 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.009, 83.009, 154.216 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.678 - 2.600 |
| R-factor | 0.1988 |
| Rwork | 0.196 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nqx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.700 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.122 | 0.676 |
| Number of reflections | 19363 | |
| <I/σ(I)> | 15.3 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15% PEG 6000, 100mM Tris buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
