3NQU
Crystal structure of partially trypsinized (CENP-A/H4)2 heterotetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2009-07-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.91983 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 61.457, 61.457, 185.619 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.23944 |
| Rwork | 0.236 |
| R-free | 0.30180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TRUNCATED CHAINS A AND B OF PDB ENTRY 1KX5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.637 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.220 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.154 | 0.634 |
| Number of reflections | 7136 | |
| <I/σ(I)> | 12.15 | 2.44 |
| Completeness [%] | 90.9 | 90.5 |
| Redundancy | 8.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 295 | 2 M ammonium sulfate, 0.1 M HEPES (pH 7.9), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
