3NQN
Crystal structure of a Protein with unknown function. (DR_2006) from DEINOCOCCUS RADIODURANS at 1.88 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97925,0.97883 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.509, 53.509, 223.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.893 - 1.880 |
| R-factor | 0.199 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.893 | 28.893 | 1.950 |
| High resolution limit [Å] | 1.880 | 4.040 | 1.880 |
| Rmerge | 0.063 | 0.022 | 0.754 |
| Number of reflections | 27005 | 5038 | 4352 |
| <I/σ(I)> | 13.56 | 45.8 | 1.3 |
| Completeness [%] | 96.3 | 97.9 | 82.8 |
| Redundancy | 6.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.0500M calcium chloride, 15.8000% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
