3NQ0
Crystal Structure of Tyrosinase from Bacillus megaterium crystallized in the absence of Zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.820, 78.700, 85.580 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 41.275 - 2.200 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nm8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.482 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 82.551 | 41.275 | 2.320 |
| High resolution limit [Å] | 2.200 | 6.960 | 2.200 |
| Rmerge | 0.032 | 0.334 | |
| Number of reflections | 29606 | ||
| <I/σ(I)> | 8.3 | 16.6 | 2.2 |
| Completeness [%] | 95.1 | 93.7 | 87.3 |
| Redundancy | 2.1 | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
