3NP4
C112D/M121E Pseudomonas aeruginosa Azurin
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.410, 55.030, 94.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.923 - 2.250 |
| R-factor | 0.21908 |
| Rwork | 0.216 |
| R-free | 0.27616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ag0 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.923 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.039 | 0.567 |
| Number of reflections | 9760 | |
| <I/σ(I)> | 8.7 | 1.3 |
| Completeness [%] | 94.0 | 94 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 25-30% PEG 4000 100 mM LiNO3 20 mM CuCl2 100 mM Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
