3NP3
C112D/M121E Pseudomonas Aeruginosa Azurin
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.860, 54.240, 94.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.280 - 2.100 |
| R-factor | 0.21677 |
| Rwork | 0.214 |
| R-free | 0.26543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ag0 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.528 | 2.154 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 7611 | |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 99.8 | 99.82 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 25-30% PEG 4000 100 mM LiNO3 20 mM CuCl2 100 mM Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
