3NOU

Light-induced intermediate structure L3 of P. aeruginosa bacteriophytochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-12
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	C 2 2 21
Unit cell lengths	154.380, 162.979, 436.098
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.000
Structure solution method	difference Fourier Method
Starting model (for MR)	3nhq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	DynamiX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Rmerge	0.106
Number of reflections	132947
Completeness [%]	99.0
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.7	293	10mg/ml protein, 0.45M ammonium phosphate, 0.1M Tris buffer, pH 7.7, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	7.7	293	10mg/ml protein, 0.45M ammonium phosphate, 0.1M Tris buffer, pH 7.7, VAPOR DIFFUSION, temperature 293K