3NOT
Light-induced intermediate structure L2 of P. aeruginosa bacteriophytochrome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97857 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 154.380, 162.979, 436.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Structure solution method | difference Fourier Method |
Starting model (for MR) | 3nhq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | DynamiX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.086 |
Number of reflections | 147934 |
Completeness [%] | 99.9 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.7 | 293 | 10mg/ml protein, 0.45M Ammonium phosphate, 0.1M Tris buffer, pH 7.7, VAPOR DIFFUSION, temperature 293K |
1 | VAPOR DIFFUSION | 7.7 | 293 | 10mg/ml protein, 0.45M Ammonium phosphate, 0.1M Tris buffer, pH 7.7, VAPOR DIFFUSION, temperature 293K |