3NOS

HUMAN ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH ARGININE SUBSTRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06-01
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	68.859, 93.264, 156.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
Rwork	0.193
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.550
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.600	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.054 *
Number of reflections	35959
<I/σ(I)>	11.8	4.5
Completeness [%]	90.0	57.8
Redundancy	6.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7	22 *	pH 6.70

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir		0.3 (M)
4	1	reservoir	2-propanol	13 (%)
5	1	reservoir	PEG3350	3 (%)