3NOM
Crystal Structure of Zymomonas mobilis Glutaminyl Cyclase (monoclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-02 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.380, 85.040, 64.860 |
| Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
| Resolution | 28.980 - 2.400 |
| R-factor | 0.2029 |
| Rwork | 0.199 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iwa |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.829 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.500 | |
| High resolution limit [Å] | 2.400 | 30.000 | 2.400 |
| Rmerge | 0.122 | 0.628 | |
| Rmeas | 0.674 | ||
| Number of reflections | 23164 | 2633 | |
| <I/σ(I)> | 14.9 | 3.4 | |
| Completeness [%] | 97.7 | 96.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
