3NOI

Crystal Structure of Natural Killer Cell Cytotoxicity Receptor NKp30 (NCR3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Detector technology	IMAGE PLATE
Collection date	2009-06-24
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	45.100, 53.020, 51.952
Unit cell angles	90.00, 107.73, 90.00

Refinement procedure

Resolution	38.815 - 1.842
R-factor	0.1664
Rwork	0.164
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.346
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES (+ EPMR)
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.790
Rmerge	0.105
Number of reflections	19666
<I/σ(I)>	150.8
Completeness [%]	97.9
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	1.6 ul of mother liquor containing 40% PEG 200, 1.4% PEG400, 10 mM CaCl2, 50 mM HEPES pH 7.4 to 2 ul of protein followed by addition of 0.4 ul of 0.1 M EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 293K