3NO5
Crystal structure of a Pfam DUF849 domain containing protein (Reut_A1631) from Ralstonia eutropha JMP134 at 1.90 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.181, 81.221, 140.429 |
| Unit cell angles | 90.00, 103.68, 90.00 |
Refinement procedure
| Resolution | 29.960 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22400 |
| Structure solution method | MR |
| Starting model (for MR) | 3chv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.530 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.961 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.124 | 0.436 |
| Number of reflections | 143033 | |
| <I/σ(I)> | 9.2 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.33 | 277 | 18.0% polyethylene glycol 8000, 0.15M magnesium acetate, 0.1M sodium cacodylate pH 6.33, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
