3NNR
Crystal structure of a TetR-family transcriptional regulator (Maqu_3571) from MARINOBACTER AQUAEOLEI VT8 at 2.49 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97934,0.97922 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.745, 58.745, 172.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.930 - 2.490 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.644 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.930 | |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 11338 | |
| <I/σ(I)> | 16.9 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 293 | 0.2000M lithium sulfate, 1.0500M potassium sodium tartrate, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
