3NND
The crystal structure of ABC transporter from Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.742, 118.427, 93.464 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 51.540 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.29600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.114 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.430 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.111 | 0.486 |
| Number of reflections | 40725 | |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 291 | 0.1 M BIS-TRIS pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
