3NND
The crystal structure of ABC transporter from Rhodopseudomonas palustris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-18 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.742, 118.427, 93.464 |
Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
Resolution | 51.540 - 2.800 |
R-factor | 0.246 |
Rwork | 0.244 |
R-free | 0.29600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.114 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 118.430 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.111 | 0.486 |
Number of reflections | 40725 | |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 7.4 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 291 | 0.1 M BIS-TRIS pH 5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |