3NMP
Crystal structure of the abscisic receptor PYL2 mutant A93F in complex with pyrabactin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.084, 105.571, 182.901 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.390 - 2.100 |
| R-factor | 0.23583 |
| Rwork | 0.234 |
| R-free | 0.26634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nmh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.555 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.093 |
| Number of reflections | 32811 |
| <I/σ(I)> | 27.6 |
| Completeness [%] | 87.0 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 2M ammonium acetate, 22% PEG 3350, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
