3NMN
Crystal structure of pyrabactin-bound abscisic acid receptor PYL1 in complex with type 2C protein phosphatase ABI1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.978, 66.710, 72.598 |
| Unit cell angles | 115.78, 95.43, 105.60 |
Refinement procedure
| Resolution | 29.620 - 2.150 |
| R-factor | 0.21301 |
| Rwork | 0.210 |
| R-free | 0.24751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kdj |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.552 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.150 |
| Rmerge | 0.098 |
| Number of reflections | 46283 |
| <I/σ(I)> | 49.1 |
| Completeness [%] | 92.0 |
| Redundancy | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.2M ammonium sulphate, 0.1M BisTris, 22% PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
