3NMA

Mutant P169S of Foot-and-mouth disease Virus RNA dependent RNA-polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-08
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	93.834, 93.834, 121.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.420 - 2.600
R-factor	0.22249
Rwork	0.221
R-free	0.25983
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u09
RMSD bond length	0.006
RMSD bond angle	0.840
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.600
Rmerge	0.103
Number of reflections	16296
<I/σ(I)>	18.4
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	35% PEG 4000, 0.2M magnesium acetate, 0.1M sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K