3NM9
HMGD(M13A)-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.750, 71.700, 89.020 |
| Unit cell angles | 92.49, 91.12, 107.10 |
Refinement procedure
| Resolution | 20.000 - 2.850 |
| R-factor | 0.24763 |
| Rwork | 0.245 |
| R-free | 0.29556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qrv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.334 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.910 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 23992 | |
| <I/σ(I)> | 21.9 | 4.6 |
| Completeness [%] | 92.3 | 82.1 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 297 | 1.2 mM HMGDM13A protein, 1.18 mM duplex DNA fragment (GCGATATCGC), 5 mM MES-Na pH 5.25, 10 mM NaCl, and 7.6% PEG 3350 equilibrated against 0.5 mL 32% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
