3NM8
Crystal structure of Tyrosinase from Bacillus megaterium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Detector technology | CCD |
Collection date | 2009-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.210, 84.490, 146.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.22516 |
Rwork | 0.220 |
R-free | 0.27280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wx2 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (1.3) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.127 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.670 |
Number of reflections | 42829 | |
<I/σ(I)> | 7.7 | 1.6 |
Completeness [%] | 98.1 | 98.4 |
Redundancy | 3.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 290 | PEG 8000, ZnAc, sodium cacodylate, pH 6.1, vapor diffusion, hanging drop, temperature 290K |