3NKG
Crystal Structure of GEBA250068378 from Sulfurospirillum deleyianum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.373, 39.414, 88.575 |
| Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
| Resolution | 44.087 - 2.000 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.21400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 25082 |
| <I/σ(I)> | 9.9 |
| Completeness [%] | 99.7 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.16 M Magnesium Acetate 0.08 M Sodium Cacodylate pH 6.5, 16% PEG 8000, 20% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
