3NKE
High resolution structure of the C-terminal domain CRISP-associated protein Cas1 from Escherichia coli str. K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.709, 90.047, 121.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.200 - 1.400 |
| R-factor | 0.17777 |
| Rwork | 0.177 |
| R-free | 0.20067 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.591 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.065 | 0.640 |
| Number of reflections | 115817 | |
| <I/σ(I)> | 28 | 2.5 |
| Completeness [%] | 99.0 | |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | O.O5 M MES 20% PEG 8k, 0.01 M Magnesium Chloride, 0.01M Adenelyl-imidodophosphate, 0.2M Ammonium sulfate, 0.3M NDSB211, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
