3NKD
Structure of CRISP-associated protein Cas1 from Escherichia coli str. K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.674, 79.300, 101.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.240 - 1.950 |
| R-factor | 0.21959 |
| Rwork | 0.217 |
| R-free | 0.25855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.240 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.095 | 0.550 |
| Number of reflections | 46873 | |
| <I/σ(I)> | 25 | 4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.6 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M bis-Tris, 0.1 M Na formate, 0.1 M Li-sulfate, 0.3M NDSB 211, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
