3NJR
Crystal structure of C-terminal domain of precorrin-6Y C5,15-methyltransferase from Rhodobacter capsulatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.011 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.354, 44.799, 84.110 |
| Unit cell angles | 90.00, 119.75, 90.00 |
Refinement procedure
| Resolution | 40.540 - 2.700 |
| R-factor | 0.18868 |
| Rwork | 0.185 |
| R-free | 0.26483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hm2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.606 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.930 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.214 |
| Number of reflections | 10402 | |
| <I/σ(I)> | 14.6 | 5.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1M tri-sodium citrate dihydrate pH 5.6, 20% isopropanol and 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
