3NJ7
1.9 A resolution X-ray structure of (GGCAGCAGCC)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-28 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.81230 |
| Spacegroup name | P 32 |
| Unit cell lengths | 47.366, 47.366, 83.209 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.553 - 1.904 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nj6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.372 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 16346 | |
| <I/σ(I)> | 18.2 | 6.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 303 | MgSO4, (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
