3NJ5

Crystal structure of chicken IL-1 hydrophobic cavity mutant 157

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	77
Detector technology	CCD
Collection date	2010-05-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	35.800, 38.666, 103.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.640 - 1.670
R-factor	0.18055
Rwork	0.178
R-free	0.23723
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.786
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.640
High resolution limit [Å]	1.670
Number of reflections	17192
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.16M (NH4)2SO4, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K