3NIX
Crystal structure of flavoprotein/dehydrogenase from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target ChR43.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-14 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.224, 244.046, 100.222 |
Unit cell angles | 90.00, 104.10, 90.00 |
Refinement procedure
Resolution | 29.331 - 2.600 |
R-factor | 0.238 |
Rwork | 0.237 |
R-free | 0.26400 |
Structure solution method | MR and SAD |
Starting model (for MR) | 3i3l |
RMSD bond length | 0.010 |
RMSD bond angle | 1.251 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.095 | 0.228 |
Number of reflections | 238952 | |
<I/σ(I)> | 21.7 | 6.3 |
Completeness [%] | 97.4 | 100 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 13% PEG 2000, 0.01M magnesium chloride, 0.005M FAD, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |