3NIU
Crystal structure of the complex of dimeric goat lactoperoxidase with diethylene glycol at 2.9 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.199, 75.587, 83.808 |
| Unit cell angles | 79.93, 77.86, 72.50 |
Refinement procedure
| Resolution | 25.000 - 2.940 |
| R-factor | 0.20069 |
| Rwork | 0.197 |
| R-free | 0.23171 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ojv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.940 |
| Number of reflections | 28536 |
| <I/σ(I)> | 4 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 280 | 0.2M Potassium dihydrogen phosphate 20%w/v PEG 3350, PH 4.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 280K |
