3NIO
Crystal structure of Pseudomonas aeruginosa guanidinobutyrase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.047, 158.954, 84.547 |
| Unit cell angles | 90.00, 114.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.23409 |
| Rwork | 0.233 |
| R-free | 0.25105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gq7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.097 |
| Number of reflections | 133054 |
| <I/σ(I)> | 24.4 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 10mM cobalt(II) chloride, 0.1M MES at pH 6.5, 1.8M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
