3NIK
The structure of UBR box (REAA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 44.534, 44.534, 139.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.570 - 1.850 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nis |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.020 | 1.850 |
| Rmerge | 0.078 | 0.045 | 0.473 |
| Number of reflections | 26430 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6.9 | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Bis-Tris pH 6.5, 10%(w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
