3NI0
Crystal Structure of Mouse BST-2/Tetherin Ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-24 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00007 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 33.685, 100.831, 110.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.154 - 1.601 |
| R-factor | 0.2149 |
| Rwork | 0.214 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d7m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.564 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.368 |
| Number of reflections | 25314 | |
| <I/σ(I)> | 28 | 4.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 0.1 M phosphate/citrate, 35% isopropanol, 5% PEG 1000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
