3NHV
Crystal Structure of BH2092 protein from Bacillus halodurans, Northeast Structural Genomics Consortium Target BhR228F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97877 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 145.873, 145.873, 145.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 2.500 |
| R-factor | 0.199 |
| Rwork | 0.195 |
| R-free | 0.24700 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.070 | 0.443 |
| Number of reflections | 73851 | |
| <I/σ(I)> | 34.97 | 3.5 |
| Completeness [%] | 97.4 | 76.5 |
| Redundancy | 6.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch, under oil | 6 | 291 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5) . Reservoir solution: 0.1M MES (pH 6), 40% PEG400, and 0.1M potassium chloride. , microbatch, under oil, temperature 291K |
