3NHH
Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-09-23 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.261, 60.121, 38.567 |
| Unit cell angles | 90.00, 94.30, 90.00 |
Refinement procedure
| Resolution | 38.460 - 2.000 |
| R-factor | 0.1973 |
| Rwork | 0.191 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BDC with water molecules and cofactors removed. Residues 23 36 111-117 truncated to ALA. All B-factors set to 20 A^2. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.1.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.120 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.169 |
| Number of reflections | 9717 | |
| <I/σ(I)> | 14 | 5.13 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.23 | 7.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 22% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
