3NHA
Nucleotide Binding Domain of Human ABCB6 (ADP Mg bound structure)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-10-15 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.815 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.272, 70.708, 71.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.130 - 2.100 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nh6 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.619 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | |
| Number of reflections | 17052 | 1125 |
| <I/σ(I)> | 14.72 | 2.4 |
| Completeness [%] | 99.5 | 97.9 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH 6.5, 40% (v/v) PEG400, 3mM ADP, 3mM Magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
