3NH6
Nucleotide Binding Domain of human ABCB6 (apo structure)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.229, 68.609, 76.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.300 - 2.000 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ghi |
RMSD bond length | 0.017 |
RMSD bond angle | 1.512 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.400 | 2.150 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.058 | |
Number of reflections | 20977 | |
<I/σ(I)> | 37.1 | 5.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15.1 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH 6.5, 40% (v/v) PEG400, vapor diffusion, hanging drop, temperature 298K |