3NH4
Crystal structure of murine aminoacylase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 62 |
| Unit cell lengths | 93.661, 93.661, 97.128 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.000 - 2.000 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gu2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.064 | 0.039 | 0.505 |
| Number of reflections | 32766 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 4.9 | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2M Sodium formate, 0.1M Sodium acetate, pH5.0, 0.1M Cesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
