3NGA
Human CK2 catalytic domain in complex with CX-4945
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.640, 127.640, 125.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 2.710 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.4) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.000 |
| High resolution limit [Å] | 2.710 |
| Number of reflections | 28836 |
| <I/σ(I)> | 34 |
| Completeness [%] | 100.0 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20-26% PEG 4000, 0.2 M ammonium sulfate, 0.1 M sodium citrate pH 6, 1 mM MgCl2 and 10 mM AMPPNP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
