3NFT
Near-atomic resolution analysis of BipD- A component of the type-III secretion system of Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.150, 60.420, 90.415 |
| Unit cell angles | 90.00, 96.01, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.510 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2izp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.269 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.710 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.067 | 0.556 |
| Number of reflections | 42898 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 5.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 35% PEG 4000, 0.1M glycine, 0.02M EDTA, 0.06M cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
