3NFQ

Crystal structure of the conserved central domain of yeast Spn1/Iws1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.9791, 0.9793, 0.9716
Spacegroup name	P 21 21 21
Unit cell lengths	63.600, 67.800, 76.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.150 - 1.850
R-factor	0.20658
Rwork	0.203
R-free	0.26456
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.710
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.051	0.380
Number of reflections	28618
<I/σ(I)>	12.1	2.8
Completeness [%]	99.6	100
Redundancy	3.02	3.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	potassium chloride, sodium thiocyanate, PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K