3NFM
Crystal Structure of the complex of type I ribosome inactivating protein with fructose at 2.5A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2010-05-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 129.850, 129.850, 40.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.920 - 2.500 |
R-factor | 0.22005 |
Rwork | 0.218 |
R-free | 0.26471 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aha |
RMSD bond length | 0.009 |
RMSD bond angle | 1.744 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.087 | 0.146 |
Number of reflections | 8728 | |
<I/σ(I)> | 7.6 | 3.9 |
Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG6000, 0.1M Sodium Phosphate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |