3NFK
Crystal structure of the PTPN4 PDZ domain complexed with the C-terminus of a rabies virus G protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.430, 53.700, 81.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.410 - 1.430 |
| R-factor | 0.1927 |
| Rwork | 0.192 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vph |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 1.510 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.068 | 0.301 |
| Number of reflections | 42795 | |
| <I/σ(I)> | 11 | 4.3 |
| Completeness [%] | 96.9 | 98.5 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 24% PEG 1500, 20% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
