3NF1

Crystal structure of the TPR domain of kinesin light chain 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-18
Detector	MAR-300
Wavelength(s)	0.92015
Spacegroup name	P 31 2 1
Unit cell lengths	74.685, 74.685, 156.168
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 2.800
R-factor	0.204
Rwork	0.200
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ceq
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.068	0.039	0.584
Number of reflections	12665
<I/σ(I)>	15.7
Completeness [%]	97.2	99.4	78.7
Redundancy	8.6	9.4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	2.0M ammonium formate, 0.1M HEPES, 0.001M TCEP., pH 7.5, vapor diffusion, sitting drop, temperature 291K